Bloom Syndrome (AA 65-114), (pThr99) antibody

Catalog No. ABIN1531494

Product Details

Target: Bloom Syndrome

Binding Specificity: AA 65-114, pThr99

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), ELISA, Western Blotting (WB), Immunofluorescence (IF)

Specificity:

Bloom Syndrome (Phospho-Thr99) Antibody detects endogenous levels of Bloom Syndrome only when phosphorylated at Thr99.

PhosphorylationH:T99

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Bloom Syndrome around the phosphorylation site of Thr99.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:5000

Comment:

Unigene-Number: Hs.169348 (NCBI Gene Symbol: BLM)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details

Target: Bloom Syndrome

Background:

Synonyms: Bloom syndrome protein, RecQ protein-like 3, DNA helicase, RecQ-like type 2, BLM, RECQ2, RECQL3

NCBI Gene Symbol: BLM

Molecular Weight: 159 kDa

Gene ID: 641

OMIM: 210900

UniProt: P54132