Retinoblastoma Protein (Rb) antibody (pThr821)
Quick Overview for Retinoblastoma Protein (Rb) antibody (pThr821) (ABIN1531516)
Target
See all Retinoblastoma Protein (Rb) (Rb Protein) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- AA 791-840, pThr821
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Specificity
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Retinoblastoma (Phospho-Thr821) Antibody detects endogenous levels of Retinoblastoma only when phosphorylated at Thr821.
PhosphorylationH:T821 M:T814 R:T792
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Purification
- The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
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Purity
- > 95 %
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Immunogen
- The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Thr821.
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Isotype
- IgG
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Application Notes
- IHC: 1:50~1:100 IF: 1:100~1:500 ELISA: 1:40000
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Comment
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Unigene-Number: Hs.408528 (NCBI Gene Symbol: RB1)
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Stable at -20°C for at least 1 year.
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Expiry Date
- 12 months
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- Retinoblastoma Protein (Rb) (Rb Protein) (Retinoblastoma Protein (Rb Protein))
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Background
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Synonyms: P105-RB, PP105, PP110, RB-1, RB1, retinoblastoma gene, Retinoblastoma-associated protein
NCBI Gene Symbol: RB1
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Molecular Weight
- 106 kDa
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Gene ID
- 5925
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OMIM
- 109800
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UniProt
- P06400
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Pathways
- Mitotic G1-G1/S Phases
Target
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