MCM4 antibody (pSer54)

Catalog No. ABIN1531581

The Rabbit Polyclonal anti-MCM4 antibody (ABIN1531581) specifically detects MCM4 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Monkey samples.

Quick Overview for MCM4 antibody (pSer54) (ABIN1531581)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-MCM4 Antibody

Binding Specificity: AA 20-69, pSer54

Purpose: MCM4 (phospho Ser54) Antibody

Specificity: Phospho-MCM4 (S54) Polyclonal Antibody detects endogenous levels of MCM4 protein only when phosphorylated at S54.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human MCM4 around the phosphorylation site of Ser54. AA range:20-69

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:20000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Alternative Name: MCM4

Background:

Synonyms: MCM4, CDC21, DNA replication licensing factor MCM4, CDC21 homolog, P1-CDC21

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. This gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks. Alternatively spliced transcri

Gene Name: MCM4

Protein Name: DNA replication licensing factor MCM4

Molecular Weight: 96 kDa

Gene ID: 4173

OMIM: 602638

UniProt: P33991

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA