BAD antibody (pSer75)

Catalog No. ABIN1531781

The Rabbit Polyclonal anti-BAD antibody has been validated for WB, ELISA and IHC. It is suitable to detect BAD in samples from Human, Mouse and Rat.

Quick Overview for BAD antibody (pSer75) (ABIN1531781)

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAD antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-BAD Antibody

Binding Specificity: AA 78-127, pSer75

Specificity:

BAD (Phospho-Ser112) Antibody detects endogenous levels of BAD only when phosphorylated at Ser112.

PhosphorylationH:S75 M:S112 R:S113

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human BAD around the phosphorylation site of Ser112.

Isotype: IgG

Application Details

Application Notes: WB: 1:500~1:1000 IHC: 1:50~1:100 ELISA: 1:5000

Comment:

Unigene-Number: Hs.370254 (NCBI Gene Symbol: BAD)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

Target Details for BAD

Target: BAD (BCL2-Associated Agonist of Cell Death (BAD))

Alternative Name: BAD

Background:

Synonyms: BAD, BBC6, Bcl- XL/Bcl-2 associated death promoter, Bcl-2 binding component 6, Bcl-2-like 8 protein, Bcl-xL/Bcl-2 associated death promoter, Bcl2-antagonist of cell death, BCL2L8

NCBI Gene Symbol: BAD

Molecular Weight: 18 kDa

Gene ID: 572

OMIM: 603167

UniProt: Q92934

Pathways: MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, Apoptosis, Fc-epsilon Receptor Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Hepatitis C, CXCR4-mediated Signaling Events