Bcl-2 antibody (pThr56)

Catalog No. ABIN1531787

The Rabbit Polyclonal anti-Bcl-2 antibody is suitable to detect Bcl-2 in samples from Human. It has been validated for WB, IHC, IF, ELISA and IP.

Quick Overview for Bcl-2 antibody (pThr56) (ABIN1531787)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA, Immunoprecipitation (IP)

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 26-75, pThr56

Purpose: Bcl-2 (phospho Thr56) Antibody

Specificity: Phospho-Bcl-2 (T56) Polyclonal Antibody detects endogenous levels of Bcl-2 protein only when phosphorylated at T56.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human BCL-2 around the phosphorylation site of Thr56. AA range:26-75

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. Immunoprecipitation: 2-5 μg:mg lysate. IF 1:200 - 1:1000. ELISA: 1:20000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: BCL-2

Background:

Synonyms: BCL2, Apoptosis regulator Bcl-2

Background: BCL2, apoptosis regulator(BCL2) Homo sapiens This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016],

Gene Name: BCL2

Protein Name: Apoptosis regulator Bcl-2

Molecular Weight: 26 kDa

Gene ID: 596

OMIM: 151430

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling