FLT3 antibody (pTyr969)

Catalog No. ABIN1532045

The Rabbit Polyclonal anti-FLT3 antibody (ABIN1532045) specifically detects FLT3 in WB, ELISA, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for FLT3 antibody (pTyr969) (ABIN1532045)

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FLT3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FLT3 Antibody

Binding Specificity: AA 935-984, pTyr969

Purpose: Flt-3 (phospho Tyr969) Antibody

Specificity: Phospho-Flt-3 (Y969) Polyclonal Antibody detects endogenous levels of Flt-3 protein only when phosphorylated at Y969.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FLT3 around the phosphorylation site of Tyr969. AA range:935-984

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:5000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for FLT3

Target: FLT3 (Fms-Related tyrosine Kinase 3 (FLT3))

Alternative Name: FLT3

Background:

Synonyms: FLT3, CD135, FLK2, STK1, Receptor-type tyrosine-protein kinase FLT3, FL cytokine receptor, Fetal liver kinase-2, FLK-2, Fms-like tyrosine kinase 3, FLT-3, Stem cell tyrosine kinase 1, STK-1, CD antigen CD135

Background: This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015],

Gene Name: FLT3

Protein Name: Receptor-type tyrosine-protein kinase FLT3

Molecular Weight: 112 kDa

Gene ID: 2322

OMIM: 136351

UniProt: P36888

Pathways: RTK Signaling