RSK1/2/3/4 (pSer221), (pSer227), (Ser218), (Ser232) antibody

Catalog No. ABIN2568448

Product Details

Target: RSK1/2/3/4

Binding Specificity: pSer221, pSer227, Ser218, Ser232

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB)

Specificity: The antibody detects endogenous levels of RSK1/2/3/4 only when phosphorylated at serine 221/227/218/232.

Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.

Immunogen: RSK1/2/3/4 (phospho Ser221/227/S218/232) antibody was raised against a peptide sequence around phosphorylation site of Serine 221/227/218/232 (A-Y-S (p)-F-C) derived from Human RSK1/2/3/4.

Isotype: IgG

Application Details

Application Notes: Western Blot: 1:500~1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store antibody at -20°C for up to one year.

Target Details

Target: RSK1/2/3/4

Background: This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway.

Molecular Weight: 85 kDa

Gene ID: 6195, 6197, 6196

NCBI Accession: NP_002944

UniProt: Q15418, P51812, Q15349, Q9UK32