Androgen Receptor antibody (pTyr363)

Catalog No. ABIN1532088

This Rabbit Polyclonal antibody specifically detects Androgen Receptor in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Androgen Receptor antibody (pTyr363) (ABIN1532088)

Target: Androgen Receptor (AR)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Androgen Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Androgen Receptor Antibody

Binding Specificity: AA 331-380, pTyr363

Purpose: AR (phospho Tyr363) Antibody

Specificity: Phospho-AR (Y363) Polyclonal Antibody detects endogenous levels of AR protein only when phosphorylated at Y363.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Androgen Receptor around the phosphorylation site of Tyr363. AA range:331-380

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. ELISA: 1:10000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Androgen Receptor

Target: Androgen Receptor (AR)

Alternative Name: Androgen Receptor

Background:

Synonyms: AR, DHTR, NR3C4, Androgen receptor, Dihydrotestosterone receptor, Nuclear receptor subfamily 3 group C member 4

Background: The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoform

Gene Name: AR

Protein Name: Androgen receptor

Molecular Weight: 98 kDa

Gene ID: 367

OMIM: 109200

UniProt: P10275

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Chromatin Binding