BAX antibody (pSer184)

Catalog No. ABIN1532125

Product Details anti-BAX Antibody

Target: BAX (BCL2-Associated X Protein (BAX))

Binding Specificity: AA 143-192, pSer184

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAX antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Specificity:

Bax (Phospho-Ser184) Antibody detects endogenous levels of Bax only when phosphorylated at Ser184.

PhosphorylationH:S184 M:S184 R:S184

Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Bax around the phosphorylation site of Ser184.

Isotype: IgG

Application Details

Application Notes: IHC: 1:50~1:100 ELISA: 1:10000

Comment:

Unigene-Number: Hs.433670, Hs.631546 (NCBI Gene Symbol: BAX)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable at -20°C for at least 1 year.

Expiry Date: 12 months

References for anti-BAX antibody (ABIN1532125)

Berger, Quast, Plötz, Kammermeier, Eberle: "Sensitization of melanoma cells for TRAIL-induced apoptosis by BMS-345541 correlates with altered phosphorylation and activation of Bax." in: Cell death & disease, Vol. 4, pp. e477, (2013) (PubMed).

Target Details for BAX

Target: BAX (BCL2-Associated X Protein (BAX))

Alternative Name: Bax (BAX Products)

Synonyms: BAX-ALPHA antibody, bax-A antibody, xBax antibody, xlbax antibody, BAX antibody, bax antibody, fj16e01 antibody, wu:fc50b10 antibody, wu:fj16e01 antibody, BCL2L4 antibody, zgc:112983 antibody, BCL2 associated X, apoptosis regulator antibody, BCL2-associated X protein L homeolog antibody, BCL2-associated X protein antibody, bcl2-associated X protein, a antibody, bcl2-associated X protein, b antibody, BAX antibody, bax.L antibody, bax antibody, baxa antibody, Bax antibody, baxb antibody

Background:

Synonyms: Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4 , BAX , BCL2L4

NCBI Gene Symbol: BAX

Molecular Weight: 21 kDa

Gene ID: 581

OMIM: 600040

UniProt: Q07812

Pathways: p53 Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity, Unfolded Protein Response