FHIT antibody (pTyr114)

Catalog No. ABIN1532181

The Rabbit Polyclonal anti-FHIT antibody is suitable to detect FHIT in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC and IF.

Quick Overview for FHIT antibody (pTyr114) (ABIN1532181)

Target: FHIT (Fragile Histidine Triad (FHIT))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FHIT antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-FHIT Antibody

Binding Specificity: AA 80-129, pTyr114

Purpose: Fhit (phospho Tyr114) Antibody

Specificity: Phospho-Fhit (Y114) Polyclonal Antibody detects endogenous levels of Fhit protein only when phosphorylated at Y114.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human FHIT around the phosphorylation site of Tyr114. AA range:80-129

Isotype: IgG

Application Details

Application Notes: IHC 1:100 - 1:300. ELISA: 1:40000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for FHIT

Target: FHIT (Fragile Histidine Triad (FHIT))

Alternative Name: FHIT

Background:

Synonyms: FHIT; Bis(5'-adenosyl)-triphosphatase; AP3A hydrolase; AP3Aase; Diadenosine 5'; 5'''-P1,P3-triphosphate hydrolase; Dinucleosidetriphosphatase; Fragile histidine triad protein

Background: This gene, a member of the histidine triad gene family, encodes a diadenosine 5',5'''-P1,P3-triphosphate hydrolase involved in purine metabolism. The gene encompasses the common fragile site FRA3B on chromosome 3, where carcinogen-induced damage can lead to translocations and aberrant transcripts of this gene. In fact, aberrant transcripts from this gene have been found in about half of all esophageal, stomach, and colon carcinomas. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Oct 2009],

Gene Name: FHIT

Protein Name: Bis(5'-adenosyl)-triphosphatase

Molecular Weight: 16 kDa

Gene ID: 2272

OMIM: 144700

UniProt: P49789