Aurora A antibody (AA 256-305)

Catalog No. ABIN1532321

This Rabbit Polyclonal antibody specifically detects Aurora A in WB, ELISA, IHC (p) and IF (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for Aurora A antibody (AA 256-305) (ABIN1532321)

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aurora A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-Aurora A Antibody

Binding Specificity: AA 256-305

Purpose: ARK-1 Antibody

Specificity: ARK-1 Polyclonal Antibody detects endogenous levels of ARK-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human Aurora Kinase. AA range:256-305

Isotype: IgG

Application Details

Application Notes: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for Aurora A

Target: Aurora A (AURKA) (Aurora Kinase A (AURKA))

Background:

Synonyms: AURKA, AIK, AIRK1, ARK1, AURA, AYK1, BTAK, IAK1, STK15, STK6, Aurora kinase A, Aurora 2, Aurora/IPL1-related kinase 1, ARK-1, Aurora-related kinase 1, hARK1, Breast tumor-amplified kinase, Serine/threonine-protein kinase 15, Serine/threonin

Background: mitogen-activated protein kinase kinase 3(MAP2K3) Homo sapiens The protein encoded by this gene is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is activated by mitogenic and environmental stress, and participates in the MAP kinase-mediated signaling cascade. It phosphorylates and thus activates MAPK14/p38-MAPK. This kinase can be activated by insulin, and is necessary for the expression of glucose transporter. Expression of RAS oncogene is found to result in the accumulation of the active form of this kinase, which thus leads to the constitutive activation of MAPK14, and confers oncogenic transformation of primary cells. The inhibition of this kinase is involved in the pathogenesis of Yersina pseudotuberculosis. Multiple alternatively spliced transcript variants that encode distinct isoforms have been reported for this gene. [provided by RefSeq, Jul 2008],

Gene Name: AURKA

Protein Name: Aurora kinase A

Molecular Weight: 45 kDa

OMIM: 603072

UniProt: O14965

Pathways: Cell Division Cycle, Asymmetric Protein Localization