POLB antibody (AA 286-335)

Catalog No. ABIN1533255

The Rabbit Polyclonal anti-POLB antibody is suitable to detect POLB in samples from Human, Rat and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for POLB antibody (AA 286-335) (ABIN1533255)

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-POLB Antibody

Binding Specificity: AA 286-335

Purpose: DNA pol β Antibody

Specificity: DNA pol β Polyclonal Antibody detects endogenous levels of DNA pol β protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human DNA Polymerase beta. AA range:286-335

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. ELISA: 1:10000.. IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for POLB

Target: POLB (Polymerase (DNA Directed), beta (POLB))

Alternative Name: DNA Polymerase beta

Background:

Synonyms: POLB, DNA polymerase beta

Background: The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011],

Gene Name: POLB

Protein Name: DNA polymerase beta

Molecular Weight: 38 kDa

Gene ID: 5423

OMIM: 174760

UniProt: P06746

Pathways: DNA Damage Repair