TDG antibody (AA 31-80)

Catalog No. ABIN1533874

The Rabbit Polyclonal anti-TDG antibody is suitable to detect TDG in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IF and IHC.

Quick Overview for TDG antibody (AA 31-80) (ABIN1533874)

Target: TDG (Thymine-DNA Glycosylase (TDG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-TDG Antibody

Binding Specificity: AA 31-80

Purpose: TDG Antibody

Specificity: TDG Polyclonal Antibody detects endogenous levels of TDG protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Purity: > 95 %

Immunogen: The antiserum was produced against synthesized peptide derived from human TDG. AA range:31-80

Isotype: IgG

Application Details

Application Notes: WB 1:500 - 1:2000. IHC 1:100 - 1:300. IF 1:200 - 1:1000. ELISA: 1:5000. Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C/1 year

Expiry Date: 12 months

Target Details for TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Alternative Name: TDG

Background:

Synonyms: TDG, G/T mismatch-specific thymine DNA glycosylase, Thymine-DNA glycosylase

Background: The protein encoded by this gene belongs to the TDG/mug DNA glycosylase family. Thymine-DNA glycosylase (TDG) removes thymine moieties from G/T mismatches by hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of DNA and the mispaired thymine. With lower activity, this enzyme also removes thymine from C/T and T/T mispairings. TDG can also remove uracil and 5-bromouracil from mispairings with guanine. This enzyme plays a central role in cellular defense against genetic mutation caused by the spontaneous deamination of 5-methylcytosine and cytosine. This gene may have a pseudogene in the p arm of chromosome 12. [provided by RefSeq, Jul 2008],

Gene Name: TDG

Protein Name: G/T mismatch-specific thymine DNA glycosylase

Molecular Weight: 46 kDa

Gene ID: 6996

OMIM: 601423

UniProt: Q13569

Pathways: DNA Damage Repair, Chromatin Binding