TEK antibody (Extracellular Domain) (Biotin)

Catalog No. ABIN155163

The Biotin-conjugated Mouse Monoclonal anti-TEK antibody (Clone Cl-2) (ABIN155163) specifically detects TEK in WB, FACS and EIA. The antibody is reactive with Human samples.

Quick Overview for TEK antibody (Extracellular Domain) (Biotin) (ABIN155163)

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TEK antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: Cl-2

Product Details anti-TEK Antibody (Biotin)

Binding Specificity: Extracellular Domain

Specificity: The unconjugated Monoclonal antibody will detect native Human TIE-2/tek in ELISA experiments and on the surface of different Human cell types.

Purification: Protein G Chromatography

Immunogen: Recombinant Human soluble extracellular domain of TIE-2

Isotype: IgG1

Application Details

Application Notes: FACS analysis and cell sorting: 2-5 μg/mL. ELISA. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore to a concentration of 50 μg/mL with sterile PBS solution containing 0.1 % BSA.

Buffer: 0.1 M Tris-Cl, 0.1 M NaCl, pH 7.4, 0.02 % Sodium Azide, 50x BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for TEK

Target: TEK (TEK Tyrosine Kinase, Endothelial (TEK))

Alternative Name: CD202b / TEK

Background: TIE2 (tyrosine kinase with Ig and EGF homology domains 2) is expressed almost exclusively in endothelial cells in mice, rats and humans. This receptor possesses a unique extracellular domain containing two immunoglobulin like loops separated by three epidermal growth factor like repeats that are connected to three fibronectin type III like repeats. The ligand for the receptor is Angiopoietin 1. Defects in TIE2 are associated with inherited venous malformations, the TIE2 signaling pathway appears to be critical for endothelial cell smooth muscle cell communication in venous morphogenesis.Synonyms: Angiopoietin-1 receptor, TIE-2, TIE2, Tunica interna endothelial cell kinase, Tyrosine-protein kinase receptor TEK, Tyrosine-protein kinase receptor TIE-2, p140 TEK

Gene ID: 7010

NCBI Accession: NP_000450

UniProt: Q02763

Pathways: RTK Signaling, Growth Factor Binding