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- Target
- PKCmy
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB)
- Specificity
- Rabbit Anti-PKCµ Polyclonal Antibody detects endogenous levels of human and mouse PKCµ protein. No cross-reactivity to other'PKC'soforms is detected.
- Cross-Reactivity (Details)
- Rabbit Anti-PKCμ Polyclonal Antibody detects endogenous levels of human and mouse PKCμ protein. No cross-reactivity to other PKC isoforms is detected.
- Purification
- Immunoaffinity chromatography
- Immunogen
- Synthetic peptide (KLH-coupled) corresponding - AA of human PKCμ protein.
- Isotype
- IgG
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- Application Notes
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.5-2 µg/mL
Other applications: user-optimized - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4, containing 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- Target
- PKCmy
- Background
- Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic, nucleotide-independent enzyme that phosphorylates serine and threonine residues. The'PKC'amily is divided into three groups depending on each the enzymes' cofactor requirements: conventional (c'PKC'soforms (alpha, betaI, betaII, and gamma) require diacylglycerol (DAG), Ca2+, and phospholipids for activation, novel (n'PKC'soforms (delta, epsilon, eta (also known as PKC-L), Theta, and µthe mouse homolog of human PKCµ(also known as PKD)) require DAG but not Ca2+ for activation, and atypical (a'PKC'soforms, (zeta, iota, and lambda, the mouse homolog of human'PKC'ota) require neither Ca2+ nor DAG. A new'PKC'ember has recently been discovered and is referred to as PKCnu.PKC's phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. These kinases also serve as major receptors for phorbol esters, a class of tumor promoters. Every member of the'PKC'amily has a specific expression profile and is believed to play a distinct role in cells.PKCµ, also known as nPKC-D1 and nPKC-mu, is a member of'PKC'amily of serine/threonine kinases. PKCµ has two characteristic cysteine-rich repeats, a conserved ATP-binding consensus sequence in the kinase domain, and the invariate aspartate essential for kinase activity.Rabbit Anti-PKCµ Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide corresponding to residues of human PKCµ protein.
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