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- Target
- Ty1 Tag
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
- Application
- Western Blotting (WB), ELISA, Immunoprecipitation (IP)
- Sequence
- EVHTNQDPLD
- Specificity
- This Antibody recognizes both N-terminal and C-terminal Ty1 tagged fusion proteins.
- Purification
- Immunoaffinity chromatography
- Immunogen
- Ty1-tag epitope peptide conjugated - KLH
- Isotype
- IgG
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anti-Ty1 Tag antibody
WB Host: Goat Polyclonal unconjugated
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- Application Notes
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Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL
Western blot: 0.2-2 µg/mLImmunoprecipitation (IP): 2-5 µg/mL
Other applications: user-optimized - Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4, containing 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
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- Target
- Ty1 Tag
- Target Type
- Tag
- Background
- Well-characterized antibodies for epitope tags are common in the study of protein expression in various systems.Rabbit Anti- Ty1-tag Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide.
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