VDAC1
Reactivity: Human
WB, IHC, ICC, IF
Host: Mouse
Monoclonal
S152B-23
PE
Application Notes
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/mL Western blot: 0.5-1 µg/mLOther Applications: user-optimized
Restrictions
For Research Use only
Format
Lyophilized
Buffer
PBS, pH 7.4, containing 0.02 % sodium azide
Preservative
Sodium azide
Precaution of Use
WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Storage
4 °C/-20 °C
Storage Comment
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Sayed, Yang, He, Pfleger, Abdellatif: "Acute targeting of general transcription factor IIB restricts cardiac hypertrophy via selective inhibition of gene transcription." in: Circulation. Heart failure, Vol. 8, Issue 1, pp. 138-48, (2015) (PubMed).
Sayed, He, Yang, Lin, Abdellatif: "Transcriptional regulation patterns revealed by high resolution chromatin immunoprecipitation during cardiac hypertrophy." in: The Journal of biological chemistry, Vol. 288, Issue 4, pp. 2546-58, (2013) (PubMed).
Voltage dependent anion selective channel protein 1 (also known as VDAC1 or mitochondrial Porin) is the predominant protein in the outer mitochondrial membrane. VDAC1 is predicted to have a beta-barrel structure and consist of either 13 or 16 membrane spanning strands. In particular, VDAC1 can function as an NADH ferricyanide reductase. It plays a vital role in modulating endostatin induced endothelial cell apoptosis. Its activity is regulated by Nek1 (NIMA related protein kinase 1) through phosphorylation of VDAC1 protein on serine 193.Rabbit Anti-VDAC1 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide within residues 150-200 of human VDAC1 (Swiss Prot: P21796).