TAP Tag antibody (HRP)

Catalog No. ABIN1574120

Product Details

Target: TAP Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), ELISA

Sequence: CSSGALDYDI PTTASENLYF Q

Specificity: Rabbit Anti-TAP-tag [HRP] Polyclonal Antibody is specific to fusion proteins containing TAP epitope tag.

Purification: Immunoaffinity chromatography

Immunogen: Synthetic peptide CSSGALDYDIPTTASENLYFQ conjugated - KLH.

Isotype: IgG1

Application Details

Application Notes: Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL.Western blot: 0.5-1 µg/mLOther Applications: user-optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4, containing 1 % BSA and 0.01 % thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains thimerosal (merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.

Target Details

Target: TAP Tag

Alternative Name: TAP-tag

Target Type: Tag

Background: Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.The TAP (tandem affinity purification) tag allows rapid purification of complexes from a relatively small number of cells without prior knowledge about the composition, activity, or function of the complex. Combined with mass spectrometry, the TAP strategy allows for the identification of proteins interacting with a given target protein. The Rabbit anti-TAP tag consists a 21 residue peptide, CSSGALDYDIPTTASENLYFQ, derived from c-terminal of the TAP construct after TEV cleavage.Rabbit Anti-TAP-tag [HRP] Polyclonal Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting fusion proteins that contain TAP tag.