Nop5p antibody
Reactivity: Saccharomyces cerevisiae
ICC, IF, IHC, WB
Host: Mouse
Monoclonal
34B12
unconjugated
1 image
-
- Target
- Nop5p
- Reactivity
- Saccharomyces cerevisiae
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blotting (WB)
- Purification
- conc. tissue culture supernatant
- Sterility
- Sterile filtered
- Clone
- 34B12
- Isotype
- IgG3
-
- Application Notes
- For western blots of yeast protein samples, use MCA-34B12 diluted 1/10,000, followed by chemiluminescent detection (ECL). For immunofluorescence on yeast cells, use MCA-34B12 diluted 1/1,000 to 1/10,000. We routinely dilute to 1/2,000. For immunoprecipitations, try 2-4 µL for the amount of lysate derived from 5 OD600 units of yeast culture. MCA-34B12 does not immunoprecipitate as well as another Nop5p monoclonal antibody, MCA-37C12, which is also available from EnCor.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- Sterile-filtered cell culture fluid from an Integra CL-350 biochamber plus sodium azide. The antibody class is IgG1 and the antibody concentration is about 1mg/mL.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 4°C short term or -20°C long term.
-
- Target
- Nop5p
- Background
- Nop5p (a.k.a. Nop58p), is localized in the nucleolus and required for pre-18S rRNA processing in Saccharomyces cerevisiae (baker's yeast).
-
You are here:
