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MYH6 antibody

MYH6 Reactivity: Human WB, ELISA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1607600
  • Target See all MYH6 Antibodies
    MYH6 (Myosin Heavy Chain 6, Cardiac Muscle, alpha (MYH6))
    Reactivity
    • 21
    • 20
    • 11
    Human
    Host
    • 29
    • 3
    Rabbit
    Clonality
    • 29
    • 3
    Polyclonal
    Conjugate
    • 15
    • 7
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MYH6 antibody is un-conjugated
    Application
    • 21
    • 10
    • 8
    • 4
    • 4
    • 2
    • 2
    • 1
    Western Blotting (WB), ELISA
    Cross-Reactivity (Details)
    Cross reactivity with myosin6 from other sources have not been determined.
    Purity
    Anti-Myosin-6 is directed against the human myosin-6 protein. The product was prepared from monospecific antiserum by delipidation and defibrination. A BLAST analysis was used to suggest reactivity with human only.
    Endotoxin Level
    Low Endotoxin : No
    Immunogen
    Anti-Myosin-6 was prepared from whole rabbit serum produced by repeated immunizations with a truncated myosin-6 construct expressed in SF9 insect cells corresponding to human myosyn-6 protein.
    Immunogen Type: Recombinant Protein
    Top Product
    Discover our top product MYH6 Primary Antibody
  • Application Notes
    Myosin-6 antibody has been tested for use in ELISA and western blot. For western blots expect a band of approximately 150 kDa in size corresponding to truncated myosin-6 protein. Specific conditions for reactivity should be optimized by the end user.
    ELISA Dilution: 1:10.000
    Western Blot Dilution: 1:1000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    25 mg/mL
    Buffer
    0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
    Handling Advice
    Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
    Storage
    -20 °C
    Storage Comment
    Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
    Expiry Date
    Expiration date is one (1) year from date of opening.
  • Target
    MYH6 (Myosin Heavy Chain 6, Cardiac Muscle, alpha (MYH6))
    Alternative Name
    Myosin 6 (MYH6 Products)
    Synonyms
    Myhca antibody, ASD3 antibody, CMD1EE antibody, CMH14 antibody, MYHC antibody, MYHCA antibody, SSS3 antibody, alpha-MHC antibody, A830009F23Rik antibody, AA517445 antibody, Myhc-a antibody, amhc antibody, MYH6 antibody, P-MHC antibody, VMHC1 antibody, myosin antibody, myosin heavy chain 6 antibody, myosin, heavy polypeptide 6, cardiac muscle, alpha antibody, myosin, heavy chain 6, cardiac muscle, alpha antibody, myosin-6 antibody, myosin, heavy chain 15 antibody, Myh6 antibody, MYH6 antibody, LOC101120580 antibody, myh6 antibody, MYH15 antibody
    Background
    Myosin VI is a myosin superfamily member with unique and intriguing features that allow it to fill a still-expanding number of cell biological roles. This actin-based motor produces force that acts towards the minus end of actin filaments, which is the opposite direction to all other characterized myosins. In mammalian cells, myosin VI is localized to endocytic vesicles, membrane ruffles, the cytosol and the Golgi complex. Its motor function is essential for several physiological functions of the cell, including normal rates of endocytosis, maintenance of Golgi morphology and protein secretion. Myosin VI regulates epithelial cell migration and plays a role in the maintenance of adhesive cellular contacts within epithelial cell layers. It is highly expressed in ovarian cancers and prostate cancers and its expression level, which is upregulated by DNA damage in a p53-dependent manner, correlates with the potential of the tumor to disseminate. More recently, myosin VI has been found involved in EGFR endocytosis through a clathrin dependent mechanism. Synonyms: MyosinVI, Unconventional myosin 6
    Gene ID
    3799
    NCBI Accession
    NP_004512
    UniProt
    P33176
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