ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Cross-Reactivity (Details)
Cross reactivity against Ferritin from other sources may occur but has not been specifically determined.
Purity
Anti-Ferritin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].
Endotoxin Level
Low Endotoxin : No
Immunogen
Ferritin [Human Spleen] Immunogen Type: Native Protein
FE
Reactivity: Human
ELISA, LF
Host: Chicken
Polyclonal
unconjugated
Application Notes
Anti-Ferritin antibody is suitable for ELISA, Western Blot and Immunohistochemistry. Expect band at 21.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user. ELISA Dilution: 1:20.000 - 1:100.000 Western Blot Dilution: 1:2.000 - 1:10.000
Restrictions
For Research Use only
Format
Liquid
Concentration
10 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Handling Advice
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Storage
-20 °C
Storage Comment
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Expiry Date
Expiration date is one (1) year from date of opening.
Ferritin Antibody detects human ferritin. Ferritin is a ubiquitous intracellular protein that stores iron and releases it in a controlled fashion. The amount of ferritin stored reflects the amount of iron stored. The protein is produced by almost all living organisms, including algae, bacteria, higher plants, and animals. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin is a globular protein complex consisting of 24 protein subunits and is the primary intracellular iron-storage protein in both prokaryotes and eukaryotes, keeping iron in a soluble and non-toxic form. Ferritin that is not combined with iron is called apoferritin. Anti-Ferritin antibody is ideal for investigators involved in enzyme and ubiquitin and UBL research. Synonyms: FTH, FTHL6