Ferritin antibody (Biotin)

Catalog No. ABIN1607712

Product Details anti-Ferritin Antibody (Biotin)

Target: Ferritin (FE)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ferritin antibody is conjugated to Biotin

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC)

Cross-Reactivity (Details): Cross reactivity against Ferritin from other sources may occur but has not been specifically determined.

Purity: Anti-Ferritin antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Ferritin [Human Spleen].

Endotoxin Level: Low Endotoxin : No

Immunogen: Ferritin [Human Spleen]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Ferritin antibody is suitable for ELISA, Western Blot and Immunohistochemistry. Expect band at 21.2 kDa in appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
ELISA Dilution: 1:20.000 - 1:100.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Ferritin

Target: Ferritin (FE)

Alternative Name: Ferritin (FE Products)

Synonyms: 1HCH antibody, BEST:LD36673 antibody, CG2216 antibody, Dmel\\CG2216 antibody, FER1 antibody, FER1HCH antibody, Fer-L antibody, Fer1 antibody, Fer1HC antibody, FerHCH antibody, HCH antibody, T21 antibody, T9 antibody, fer1 antibody, fer1hch antibody, l(3)00451 antibody, l(3)j10B4 antibody, SFerH-1 antibody, SOF-35 antibody, SOF-5L antibody, NV13011 antibody, ACP1 antibody, F8A5.24 antibody, F8A5_24 antibody, Ferritin 1 heavy chain homologue antibody, ferritin light chain antibody, ferritin antibody, chromosome 26 open reading frame, human C10orf131 antibody, hyaluronan and proteoglycan link protein 2 antibody, NAD(P)-linked oxidoreductase superfamily protein antibody, Fer1HCH antibody, H-1 antibody, YPDSF_1340 antibody, ftnA antibody, Ft antibody, C26H10orf131 antibody, HAPLN2 antibody, AT1G60730 antibody

Background: Ferritin Antibody detects human ferritin. Ferritin is a ubiquitous intracellular protein that stores iron and releases it in a controlled fashion. The amount of ferritin stored reflects the amount of iron stored. The protein is produced by almost all living organisms, including algae, bacteria, higher plants, and animals. In humans, it acts as a buffer against iron deficiency and iron overload. Ferritin is a globular protein complex consisting of 24 protein subunits and is the primary intracellular iron-storage protein in both prokaryotes and eukaryotes, keeping iron in a soluble and non-toxic form. Ferritin that is not combined with iron is called apoferritin. Anti-Ferritin antibody is ideal for investigators involved in enzyme and ubiquitin and UBL research. Synonyms: FTH, FTHL6

Gene ID: 2495

UniProt: P02794

Pathways: Transition Metal Ion Homeostasis