GLB1 antibody (Tag) (Biotin)

Catalog No. ABIN1607765

Product Details anti-GLB1 Antibody (Biotin)

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Binding Specificity: Tag

Reactivity: Please inquire

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLB1 antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against β-Galactosidase from other sources may occur but have not been specifically determined.

Purity: Anti-BETA GALACTOSIDASE Antibody, Biotin Conjugated, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified beta-Galactosidase [E.coli].

Endotoxin Level: Low Endotoxin : No

Immunogen: Beta Galactosidase (E.coli)
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Suitable for immunoblotting, ELISA, immunohistochemistry, immunomicroscopy as well as other antibody based assays using streptavidin or avidin conjugates requiring lot-to-lot consistency.  The antibody recognizes both frozen tissue sections, paraffin embedded tissue and 4% paraformaldehyde fixed tissue for most immunohistochemical analysis. A 1:5.000 dilution has been reported to be successful for staining by immunoblot of beta-galactosidase fusion proteins after transfer using a semi-dry transfer apparatus.  A 1:1.500 dilution has been reported to detect beta-galactosidase in adult rat spinal cord tissue after infection with helper-dependent adenovirus expressing lacZ.  In this particular experiment, tissue was perfused with 4% paraformaldehyde and cryostat-cut (35 µm) to produce free-floating sections.  A 1:5.000 dilution has been reported to be successful for staining of brain sections from transgenic mice expressing nuclear beta-galactosidase when assayed by immunofluorescence microscopy.  A 1:5.000 dilution has been reported for immunofluorescent staining of methanol fixed, devitellinized Drosophila embryos.  Although a wide range of conditions was reported to be effective, a 1:10.000 dilution was noted to show no background and to be suitable for double labeling experiments.  Optimal titers for other applications should be determined by the researcher.
ELISA Dilution: 1:10.000 - 1:50.000
Western Blot Dilution: 1:2.000 - 1:10.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for GLB1

Target: GLB1 (Galactosidase, beta 1 (GLB1))

Alternative Name: Beta Galactosidase (GLB1 Products)

Synonyms: EBP antibody, ELNR1 antibody, MPS4B antibody, DER antibody, XR antibody, 0610038K04Rik antibody, 1810027P18Rik antibody, glb antibody, CG9092 antibody, Dmel\\CG9092 antibody, beta-GAL antibody, beta-Gal-1 antibody, beta-gal antibody, gal antibody, lacZ-1 antibody, AW125515 antibody, Bge antibody, Bgl antibody, Bgl-e antibody, Bgl-s antibody, Bgl-t antibody, Bgs antibody, Bgt antibody, C130097A14Rik antibody, BGAL antibody, zgc:110823 antibody, ECK0341 antibody, JW0335 antibody, GLB antibody, galactosidase beta 1 antibody, dicarbonyl and L-xylulose reductase antibody, dicarbonyl L-xylulose reductase antibody, beta-D-galactosidase antibody, beta-galactosidase antibody, beta galactosidase antibody, galactosidase, beta 1 antibody, beta-galactosidase-like antibody, GLB1 antibody, DCXR antibody, Dcxr antibody, lacZ antibody, SSP_RS00525 antibody, lacH antibody, ECL_03691 antibody, Gal antibody, Glb1 antibody, glb1 antibody, VV2_1327 antibody, LOC100056371 antibody

Background: Anti Beta Galactosidase Antibody recognizes the enzyme beta galactosidase, or β-galactosidase, that is a component of assays used frequently in genetics, molecular biology (see X-gal) for a blue white screen, and other life sciences. IPTG induces production of β-galactosidase by binding and inhibiting the lac repressor. Since it is highly expressed and accumulated in lysosomes in senescent cells, it is used as a senescence biomarker both in vivo and in vitro in qualitative and quantitative assays, despite its limitations.

Pathways: Glycosaminoglycan Metabolic Process