Trypsin Inhibitor antibody (Biotin)

Catalog No. ABIN1607812

Product Details anti-Trypsin Inhibitor Antibody (Biotin)

Target: Trypsin Inhibitor

Reactivity: Soybean

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Trypsin Inhibitor antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA

Cross-Reactivity (Details): Cross reactivity against Trypsin Inhibitor from other sources is unknown.

Purity: Anti-Trypsin Inhibitor antibody is an IgG fraction purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Trypsin Inhibitor [Soy Bean].

Endotoxin Level: Low Endotoxin : No

Immunogen: Anti-Trypsin Inhibitor was produced by repeated immunizations with soy bean trypsin inhibitor.
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-Trypsin Inhibitor antibody is suitable for western blotting and for capture ELISA. Researchers should determine optimal titers for applications that are not stated below.
ELISA Dilution: 1:5.000 - 1:25.000
Western Blot Dilution: 1:500 - 1:3.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for Trypsin Inhibitor

Target: Trypsin Inhibitor

Abstract: Trypsin Inhibitor Products

Synonyms: CRISP11 antibody, LCRISP2 antibody, GRMZM2G447785 antibody, cysteine rich secretory protein LCCL domain containing 2 antibody, trypsin inhibitor antibody, trophoblast Kunitz domain protein 1 antibody, Kazal-type serine proteinase inhibitor 1 antibody, Bowman-Birk type bran trypsin inhibitor antibody, CRISPLD2 antibody, LOC548021 antibody, TKDP1 antibody, Spi1 antibody, LOC100284348 antibody

Background: Anti-Trypsin Inhibitor detects trypsin inhibitor from soy bean. Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. Soybean trypsin inhibitor will also inhibit other proteases by a mechanism similar to trypsin. It has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. However, Soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases. Anti-Trypsin Inhibitor Antibody is ideal for investigators involved in Cell Signaling, Biochemistry and Signal Transduction research. Synonyms: 25 kDa trypsin inhibitor (Protease inhibitor 15 preproprotein) antibody, CRISP8 antibody, KTi antibody, Kti S antibody, KTi3 antibody, KtiS antibody, Kunitz trypsin inhibitor antibody, Kunitz type trypsin inhibitor A antibody

Gene ID: 547831

UniProt: P01070