Urease antibody (Biotin)
URE
Reactivity: Jack Bean
WB, ELISA
Host: Rabbit
Polyclonal
Biotin
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- Target See all Urease (URE) products
- Urease (URE)
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Reactivity
- Jack Bean
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This Urease antibody is conjugated to Biotin
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Application
- Western Blotting (WB), ELISA
- Cross-Reactivity (Details)
- Cross reactivities against Urease from other sources may occur but have not been specifically determined.
- Purity
- Anti-Urease antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Urease [Jack Bean].
- Endotoxin Level
- Low Endotoxin : No
- Immunogen
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Urease [Jack Bean]
Immunogen Type: Native Protein - Isotype
- IgG
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anti-Urease (URE) antibody
URE Reactivity: Jack Bean WB, ELISA, IP Host: Rabbit Polyclonal unconjugated
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- Application Notes
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Anti-Urease antibody has been assayed against 1.0 µg of Urease in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:4.000 to 1:20.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
Western Blot Dilution: 1:500 - 1:5.000 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
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0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free - Handling Advice
- Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
- Expiry Date
- Expiration date is one (1) year from date of opening.
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- Target
- Urease (URE)
- Alternative Name
- Urease (URE Products)
- Synonyms
- F12B7.10 antibody, F12B7_10 antibody, urease antibody, urease antibody, URE antibody, CNH01900 antibody
- Background
- Urease is a protein that is commonly secreted from the bacterium H. Pylori and is integral to the immune response within the gastric system. Urease carries out a key enzymatic reaction in converting urea into free ammonia and carbonic acid. Urease is commonly derived from the Jack Bean plant in scientific research as the amino acid sequences are highly conserved between species and it is an abundant source of the protein. Anti-Urease Antibody is ideal for investigators in Immunology, Enzymology, and Cell Biology research. Synonyms: Urea Amidohydrolase antibody
- UniProt
- P07374
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