D Amino Acid Oxidase antibody (Biotin)

Catalog No. ABIN1607835

Product Details anti-D Amino Acid Oxidase Antibody (Biotin)

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Reactivity: Pig

Host: Sheep

Clonality: Polyclonal

Conjugate: This D Amino Acid Oxidase antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Cross-Reactivity (Details): Cross reactivities against D-Amino Acid Oxidase from other sources may occur but have not been specifically determined.

Purity: Anti-D-Amino Acid Oxidase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney].

Endotoxin Level: Low Endotoxin : No

Immunogen: D-Amino Acid Oxidase [Pig Kidney]
Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes: Anti-D-Amino Acid Oxidase has been assayed against 1.0 µg of D-Amino Acid Oxidase in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:16.000 to 1:68.000 of the reconstitution concentration is suggested for this product.
ELISA Dilution: 1:5.000 - 1:20.000
IF Immunoprecipitation Dilution: 1:100
Western Blot Dilution: 1:500 - 1:5.000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Handling Advice: Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Storage: -20 °C

Storage Comment: Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.

Expiry Date: Expiration date is one (1) year from date of opening.

Target Details for D Amino Acid Oxidase

Target: D Amino Acid Oxidase (DAO) (D-Amino-Acid Oxidase (DAO))

Alternative Name: D-Amino Acid Oxidase (DAO Products)

Synonyms: DAAO antibody, DAMOX antibody, OXDA antibody, daao antibody, oxda antibody, damox antibody, DAO antibody, Dao1 antibody, DKFZp469N2232 antibody, AI987963 antibody, Dao-1 antibody, SC5F2A.23c antibody, wu:fb55b11 antibody, zgc:113922 antibody, ABP antibody, ABP1 antibody, DAO1 antibody, KAO antibody, MGC114783 antibody, Abp antibody, Abp1 antibody, D-amino acid oxidase antibody, D-amino-acid oxidase antibody, D-amino-acid oxidase, tandem duplicate 1 antibody, amine oxidase, copper containing 1 antibody, D-amino-acid oxidase L homeolog antibody, DAO antibody, Dao antibody, dao antibody, SCO6740 antibody, dao.1 antibody, AOC1 antibody, PTRG_02224 antibody, SJAG_02496 antibody, dao.L antibody, Aoc1 antibody

Background: Anti-D-Amino Acid Oxidase antibody is part of the DAMOX/DASOX family and resides in the peroxisome. D-Amino Acid Oxidase modulates the level of the neuromodulater D-serine in the brain, contributes to dopamine synthesis, and has a high activity towards D-DOPA. It could act to remove D-amino acids that accumulate with age. Anti-D-Amino Oxidase prefers small hydrophobic side chains, then bearing polar, aromatic, and basic groups. It does not act on acidic amino acids. Anti-D-Amino Acid Oxidase is ideal for investigators interested in Metabolism, Signal Transduction, and Tags & Cell Marker research. Synonyms: D-amino-acid oxidase DAMOX DAAO DAO EC=1.4.3.3

Gene ID: 397134

UniProt: P00371