ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Cross-Reactivity (Details)
Cross reactivity against Albumin from other sources may occur but have not been specifically determined.
Purification
Bovine serum antibody was purified as an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Albumin (Bovine Serum].
Endotoxin Level
Low Endotoxin : No
Immunogen
Albumin (Bovine Serum) Immunogen Type: Native Protein
Anti-BSA antibody is suitable for detection of bovine serum albumin in biological samples by ELISA, western blot and immunohistochemisty assays. Specific dilutions are recommended but should be opitmized by researcher. ELISA Dilution: 1:50.000 Immunohistochemistry Dilution: 1:300 - 1:2.500 Western Blot Dilution: 1:1.000 - 1:5.000
Restrictions
For Research Use only
Format
Liquid
Concentration
10 mg/mL
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 Stabilizer: 10 mg/mL Polyethylene Glycol (PEG-8000)
Handling Advice
Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Storage
-20 °C
Storage Comment
Store vial at -20 °C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
Expiry Date
Expiration date is one (1) year from date of opening.
PRO0883 antibody, PRO0903 antibody, PRO1341 antibody, albumin antibody, ALB antibody
Background
Anti-BSA antibody is specific for the full-length BSA precursor protein is 607 amino acids in length. An N-terminal 18-residue signal peptide is cut off from the precursor protein upon secretion, and an additional 4 amino acids is cleaved to yield the mature BSA protein that contains 585 amino acids. BSA has numerous biochemical applications including ELISAs (Enzyme-Linked Immunosorbent Assay), immunoblots, and immunohistochemistry, and it is also used as a nutrient in cell and microbial culture. BSA is used as a stabilizer for some proteins and enzymes. This protein does not affect other enzymes that do not need it for stabilization. Synonyms: ALB antibody, Albumin antibody, Allergen Bos d 6 antibody, BSA antibody, DKFZp779N1935 antibody