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SYT7 antibody (AA 150-239)

SYT7 Reactivity: Mouse WB, IHC, ICC, IF Host: Mouse Monoclonal S275-14 unconjugated
Catalog No. ABIN1686633
  • Target See all SYT7 Antibodies
    SYT7 (Synaptotagmin VII (SYT7))
    Binding Specificity
    • 11
    • 8
    • 8
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 150-239
    Reactivity
    • 31
    • 26
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Mouse
    Host
    • 29
    • 15
    • 1
    Mouse
    Clonality
    • 30
    • 15
    Monoclonal
    Conjugate
    • 18
    • 4
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This SYT7 antibody is un-conjugated
    Application
    • 37
    • 17
    • 15
    • 11
    • 10
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Specificity
    Detects ~45 kDa. Does not cross-react with Synaptotagmin-6 (or others). Can identify other isoforms bands at ~65kD.
    Cross-Reactivity
    Human, Mouse, Rat
    Purification
    Protein G Purified
    Immunogen
    Fusion protein amino acids 150-239 (Cytoplasmic C2A domain) of mouse Synaptotagmin-7
    Clone
    S275-14
    Isotype
    IgG2b
    Top Product
    Discover our top product SYT7 Primary Antibody
  • Application Notes
    • WB (1:1000)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    Comment

    1 μg/ml of ABIN1686633 was sufficient for detection of Synaptotagmin-7 in 20 μg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    -20 °C
    Storage Comment
    -20°C
  • Target
    SYT7 (Synaptotagmin VII (SYT7))
    Alternative Name
    Synaptotagmin 7 (SYT7 Products)
    Synonyms
    CG10322 antibody, CG2381 antibody, Dmel\\CG2381 antibody, SYT7 antibody, Syt 7 antibody, Syt VII antibody, dsyt7 antibody, syt7 antibody, MGC145945 antibody, IPCA-7 antibody, IPCA7 antibody, PCANAP7 antibody, SYT-VII antibody, SYTVII antibody, AI851541 antibody, B230112P13Rik antibody, Synaptotagmin 7 antibody, synaptotagmin VII antibody, synaptotagmin 7 antibody, Syt7 antibody, SYT7 antibody, syt7 antibody
    Background
    Synaptotagmins constitute a family of membrane-trafficking proteins that are characterized by an N-terminal transmembrane region (TMR), a variable linker, and two C-terminal C2 domains - C2A and C2B. There are 15 members in the mammalian synaptotagmin family. There are several C2-domain containing protein families that are related to synaptotagmins, including transmembrane (Ferlins, E-Syts, and MCTPs) and soluble (RIMs, Munc13s, synaptotagmin-related proteins and B/K) proteins. The synaptotagmins are integral membrane proteins of synaptic vesicles thought to serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Calcium binding to synaptotagmin participates in triggering neurotransmitter release at the synapse. The first domain mediates Ca(2+)-dependent phospholipid binding. The second C2 domain mediates interaction with Stonin 2. Synaptotagmin may have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacylbackbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner, these are neurexins, syntaxin and AP2.
    Gene ID
    54525
    NCBI Accession
    NP_061271
    UniProt
    Q9R0N7
    Pathways
    Synaptic Vesicle Exocytosis
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