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pan Keratin antibody

panKRT Reactivity: Human IHC (p), IHC (fro) Host: Mouse Monoclonal B311-1 unconjugated

Catalog No. ABIN966802

Datasheet as PDF

Target See all pan Keratin (panKRT) Antibodies

pan Keratin (panKRT)
Reactivity
82

58

39

34

22

20

20

19

19

15

15

14

10

6

1

1

1
Human
Host
54

34

1
Mouse
Compatible Secondaries
Clonality
57

32
Monoclonal
Conjugate
33

6

4

4

4

4

4

4

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This pan Keratin antibody is un-conjugated
Application
55

54

49

24

19

16

14

14

7

7

6

5

3

2
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity

This antibody recognizes keratin polypeptides with molecular weights of 59, 58, 56, 52.5, 56.5, 54 and 45 kD, which correspond tocytokeratins 4,5,6,8,10,13 and 18.

Purification

Concentrated.

Immunogen

Keratin fraction isolated from cultured cell line A-431, epidermoid carcinoma of vulva.

Clone

B311-1

Isotype

IgG1 kappa

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anti-pan Keratin (panKRT) antibody
panKRT Reactivity: Mammalian FACS, WB, IHC (p), ICC, IP Host: Mouse Monoclonal C-11 unconjugated

anti-pan Keratin (panKRT) antibody
panKRT Reactivity: Human, Rat, Mouse WB, IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

anti-pan Keratin (panKRT) antibody
panKRT Reactivity: Human WB, IHC, ELISA, ICC Host: Mouse Monoclonal 7H8C4 unconjugated

anti-pan Keratin (panKRT) antibody (FITC)
panKRT Reactivity: Mammalian FACS Host: Mouse Monoclonal C-11 FITC

anti-pan Keratin (panKRT) (AA 331-430) antibody
panKRT Reactivity: Human, Rat IHC (p), ICC, IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-pan Keratin (panKRT) (pan) antibody
panKRT Reactivity: Human, Rat, Mouse WB, IHC (p), ELISA Host: Rabbit Polyclonal unconjugated

anti-pan Keratin (panKRT) antibody (PE)
panKRT Reactivity: Mammalian FACS Host: Mouse Monoclonal C-11 PE

anti-pan Keratin (panKRT) antibody (APC)
panKRT Reactivity: Mammalian FACS Host: Mouse Monoclonal C-11 APC

anti-pan Keratin (panKRT) antibody (Biotin)
panKRT Reactivity: Mammalian FACS, WB, IHC (p), ICC, IP Host: Mouse Monoclonal C-11 Biotin

anti-pan Keratin (panKRT) antibody
panKRT Reactivity: Human IHC Host: Rabbit Monoclonal MSVA-000R unconjugated Recombinant Antibody

Application Notes

IHC (FFPE): 1:50-1:100 (Requires high temp. antigen demaking, 10 mM Citrate pH 6.0 boiling tissue for 10-20 mins. followed by cooling at RT for 10-20 mins or protease digestion).
Western blotting: 1:100-1:400
Immunoprecipitation.
Staining Procedure: This antibody can be used on frozen tissue sections and on formalin-fixed, paraffin-embedded tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC, with High temperature antigenic unmasking technique predigestion is recommended with this antibody when using paraffin-embedded tissue sections.

Comment

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Skin, Colonic Carcinoma

Restrictions

For Research Use only
Format

Liquid

Concentration

0.35 mg/ml.

Buffer

20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C
Target

pan Keratin (panKRT)

Alternative Name

Pan Cytokeratin, Pan CK-II (panKRT Products)

Synonyms

xk70a antibody, keratin antibody, keratin S homeolog antibody, LOC431321 antibody, krt.S antibody

Molecular Weight

45, 52.5 ,54, 56, 56.5, 58, & 59 kDa