ODF2 antibody (AA 1-100) (Alexa Fluor 488)

Catalog No. ABIN1694608

Product Details anti-ODF2 Antibody (Alexa Fluor 488)

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Binding Specificity: AA 1-100

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ODF2 antibody is conjugated to Alexa Fluor 488

Application: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Rat

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ODF2/Cenexin1

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ODF2

Target: ODF2 (Outer Dense Fiber of Sperm Tails 2 (ODF2))

Alternative Name: ODF2/Cenexin1 (ODF2 Products)

Synonyms: odf84 antibody, odf2/1 antibody, odf2/2 antibody, DKFZp469J0214 antibody, CT134 antibody, ODF2/1 antibody, ODF2/2 antibody, ODF84 antibody, si:dkey-228b2.4 antibody, AI848335 antibody, MMTEST29 antibody, outer dense fiber of sperm tails 2 antibody, outer dense fiber of sperm tails 2a antibody, outer dense fiber of sperm tails 2 L homeolog antibody, ODF2 antibody, odf2 antibody, odf2a antibody, odf2.L antibody, Odf2 antibody

Background:

Synonyms: sperm tail structural protein, 84 kDa outer dense fiber protein, Cenexin 1, Cenexin1 variant 1, KKT 4, KKT4, ODF 2, ODF 84, ODF2, ODF2/1, ODF2/2, ODF84, Outer dense fiber of sperm tail 2, Outer dense fiber of sperm tails 2, outer dense fiber of sperm tails, Outer dense fiber of sperm tails protein 2, Outer dense fiber of sperm tails, 84 kD, Outer dense fiber protein 2, Sperm outer dense fiber major protein 2, Testis specic autoantigen, ODFP2_HUMAN.

Background: Cenexin1 is an isoform of ODF2, that unlike ODF2 is present in several somatic cell types. Cenexin1 acts as a general scaffold protein that is specifically localised to the distal/subdistal appendages of mother centrioles. Cenexin1 is required for proper localization of Plk1 to the centrosomes. This centrosomal localization of Plk1 is required for proper microtubule function. Cenexin1 recruits Plk1 via a C-terminal extension of cenexin1 that is not present in ODF2. Cenexin1 is required for proper mitotic progression, depletion of Cenexin1 µLtimately leads to chromosome missegregation and apoptosis. The ODF2 (outer dense fiber 2) gene encodes both ODF2 and Cenexin1, which have very different functions. ODF2 is a major component of sperm tail outer dense fibers (ODFs). ODFs are filamentous structures located on the outside of the axoneme in the midpiece and principal piece of the mammalian sperm tail. They may help to maintain the passive elastic structures and elastic recoil of the sperm tail, and may also modulate sperm motility.

Gene ID: 4957

Pathways: M Phase