ECH1 antibody (AA 21-120) (HRP)

Catalog No. ABIN1711290

Product Details anti-ECH1 Antibody (HRP)

Target: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Binding Specificity: AA 21-120

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ECH1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human ECH1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ECH1

Target: ECH1 (Enoyl Coenzyme A Hydratase 1, Peroxisomal (ECH1))

Alternative Name: ECH1 (ECH1 Products)

Synonyms: HPXEL antibody, Pxel antibody, AA617331 antibody, enoyl-CoA hydratase 1, peroxisomal antibody, enoyl CoA hydratase 1, peroxisomal antibody, enoyl-CoA hydratase 1 antibody, delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial antibody, enoyl-CoA hydratase 1, peroxisomal L homeolog antibody, enoyl coenzyme A hydratase 1, peroxisomal antibody, ech1 antibody, ECH1 antibody, LOC747511 antibody, ech1.L antibody, Ech1 antibody

Background:

Synonyms: 4-dienoyl-CoA isomerase, 5-Delta2, Delta3 5 Delta2 4 dienoyl CoA isomerase, Delta3 5 Delta2 4 dienoyl CoA isomerase mitochondrial, Delta3, Delta3 5 delta2 4 dienoyl CoA isomerase, Dienoyl CoA isomerase, ECH 1, Ech1, ECH1_HUMAN, Enoyl Coenzyme A hydratase 1, Enoyl Coenzyme A hydratase 1 peroxisomal, HPXEL, mitochondrial, MS1034, Peroxisomal enoyl CoA hydratase 1, Peroxisomal enoyl coenzyme A hydratase like protein.

Background: ECH1 is a 328 amino acid protein that localizes to both the mitochondrion and the peroxisome and belongs to the hydratase/isomerase superfamily. Existing as a homohexamer, ECH1 is involved in the fatty acid-beta oxidation pathway, specifically functioning to catalyze the isomerization of 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA. The gene encoding ECH1 maps to human chromosome 19, which is the genetic home for a number of immunoglobulin superfamily members, including the killer cell and leukocyte Ig-like receptors, a number of ICAMs, the CEACAM and PSG family and Fc receptors (FcRs).

Gene ID: 1891

Pathways: Monocarboxylic Acid Catabolic Process