HIC1 antibody (AA 501-650) (HRP)

Catalog No. ABIN1711710

Product Details anti-HIC1 Antibody (HRP)

Target: HIC1 (Hypermethylated in Cancer 1 (HIC1))

Binding Specificity: AA 501-650

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIC1 antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HIC1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HIC1

Target: HIC1 (Hypermethylated in Cancer 1 (HIC1))

Alternative Name: HIC1 (HIC1 Products)

Synonyms: zfHIC1 antibody, HIC1 antibody, ZBTB29 antibody, ZNF901 antibody, hic-1 antibody, AA408311 antibody, HIC-1 antibody, hypermethylated in cancer 1 antibody, HIC ZBTB transcriptional repressor 1 antibody, hic1 antibody, HIC1 antibody, Hic1 antibody

Background:

Synonyms: Hic 1, Hic-1, Hic1, HIC1_HUMAN, Hypermethylated in cancer 1, Hypermethylated in cancer 1 protein, ZBTB29, Zinc finger and BTB domain-containing protein 29, ZNF901.

Background: Hypermethylated in cancer (HIC-1) was originally identified as a target of p53-induced gene expression. HIC-1 is deleted in the genetic disorder Miller-Dieker syndrome (MDS), and the expression of HIC-1 is also frequently suppressed in leukemia and various cancers due to the hypermethylation of specific DNA regions and the resulting transcriptional silencing. These and other studies indicate that HIC-1 acts as a putative tumor suppressor protein that mediates transcriptional repression. HIC-1 is ubiquitously expressed in adult tissues and its structure is defined by five zinc fingers and an N-terminal broad complex POZ (or BTB) domain. In several BTB/POZ containing proteins, including BCL-6 and the promyelocytic leukemia zinc-finger (PLZF) oncoprotein, this domain interacts with the SMRT/N-CoR-mSin3A HDAC complex and is directly involved in repressing and silencing gene transcription. When this domain is deleted, as with the oncogenic PLZF-RAR chimera of promyelocytic leukemias, this transcriptional repression is attenuated. Conversely, HIC-1 does not interact with components of the HDAC complex, suggesting that HIC-1-induced transcriptional repression is unassociated with the POZ/BTB domain.

Gene ID: 3090

Pathways: Positive Regulation of Response to DNA Damage Stimulus