Reacts with human, mouse and rat TATA Binding Protein. It does not react with TBP from Drosophila, yeast, silk worm, or Xenopus.
Purification
Purified (protein G)
Immunogen
Recombinant human TBP (epitope within amino acid residues 1-20 of human, mouse and rat TBP. The specific epitope for this antibody is accessible when the C-terminal domain of TBP is removed or when TBP is complexed with DNA.)
Working dilution: Optimal dilution should be determined by the end user. The following are guidelines only: WB(2 μg/mL- ELISA (1:100 - 1:2000) Gel Super Shift Assays (1:1 - 1:100) IP(1:10 - 1:500) ChIP(5 - 10 μg for 25 μg of chromatin.)
Restrictions
For Research Use only
Buffer
PBS, pH 7.4
Handling Advice
Dilute in PBS or medium that is identical to that used in the assay system.
Storage
-20 °C/-80 °C
Storage Comment
Stable for at least one year at -20°C to -70°C. Avoid multiple freeze-thaw cycles.