VAMP1, 2, 3 (AA 1-81) antibody

Catalog No. ABIN1742191

Product Details

Target: VAMP1, 2, 3

Binding Specificity: AA 1-81

Reactivity: Mouse, Rat, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC), Immunoprecipitation (IP)

Specificity: Specific for all three isoforms VAMP 1, 2 and 3.

Purification: antiserum

Immunogen: Recombinant protein of the cytoplasmic part of rat cellubrevin (aa 1-81).

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
ICC: 1 : 500
IHC: not tested yet

Comment:

ICC: Methanol and acetone fixation, see Annaert et al., 1997 This antibody is recommended for ICC of non-neuronal cells. This antibody detects the tetanus and Botulinum B toxin cleavage product in toxin treated tissue homogenates.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS

Handling Advice: Crude antisera are more robust than monoclonals. With anti-microbials added, they may be stored at 4 °C.
Serum does not contain active proteases, in fact, serum itself contains a powerful cocktail of protease inhibitors. Frozen storage (-20 °C),however, is preferable.

Storage: 4 °C/-20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References

Tobin, Schwab, Lelos, Onaka, Pittman, Ludwig: "Expression of exocytosis proteins in rat supraoptic nucleus neurones." in: Journal of neuroendocrinology, Vol. 24, Issue 4, pp. 629-41, (2012) (PubMed).

Target Details

Target: VAMP1, 2, 3

Background: Synonyms: VAMP 3