VAMP1, 2, 3 (AA 1-81) antibody

Catalog No. ABIN1742193

Product Details

Target: VAMP1, 2, 3

Binding Specificity: AA 1-81

Reactivity: Mouse, Rat, Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: Specific for all three isoforms VAMP 1, 2 and 3.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Recombinant protein of the cytoplasmic part of rat cellubrevin (aa 1-81).

Application Details

Application Notes: WB: 1 : 1000 up to 1 : 5000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: 1 : 200

Comment:

ICC: Methanol and acetone fixation, see Annaert et al., 1997 This antibody is recommended for ICC of non-neuronal cells. This antibody detects the tetanus and Botulinum B toxin cleavage product in toxin treated tissue homogenates.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References

Arsenault, Cuijpers, Ferrari, Niranjan, Rust, Leese, OBrien, Binz, Davletov: "Botulinum protease-cleaved SNARE fragments induce cytotoxicity in neuroblastoma cells." in: Journal of neurochemistry, Vol. 129, Issue 5, pp. 781-91, (2014) (PubMed).

Target Details

Target: VAMP1, 2, 3

Background: Synonyms: VAMP 3