Homer 1b/c (AA 152-354) antibody

Catalog No. ABIN1742339

Product Details

Target: Homer 1b/c

Binding Specificity: AA 152-354

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunocytochemistry (ICC)

Specificity: Specific for homer 1b and 1c

Cross-Reactivity (Details): no cross-reactivity to homer 1a.

Purification: Affinity purified with the immunogen. Rabbit serum albumin was added for stabilization.

Immunogen: Purified recombinant protein (aa 152-354 of human homer1b)

Application Details

Application Notes: WB: 1 : 1000 (AP staining)
IP: not tested yet
ICC: 1 : 500
IHC: not tested yet

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 50 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS

Handling Advice: Affinity purified antibodies are less robust than antisera, since protease inhibitors are also removed during purification. Hence, storage at 4 °C for prolonged periods (i.e. several weeks), is not recommended.

Storage: -20 °C

Storage Comment: Unlabeled lyophilized antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4°C for several years. Lyophilized antibodies must not be stored in the freezer, they may be destroyed!

References

Yoon, Piguel, Khalatyan, Dionisio, Savas, Penzes: "Homer1 promotes dendritic spine growth through ankyrin-G and its loss reshapes the synaptic proteome." in: Molecular psychiatry, (2021) (PubMed).

Kim, Kong, Yamamoto, Kuramoto, Huang, Wang, Hong, Xiao, Levine, Qiu, Zhao, Miller, Dong, Meltzer, Xu, He: "An autophagy-related protein Becn2 regulates cocaine reward behaviors in the dopaminergic system." in: Science advances, Vol. 7, Issue 8, (2021) (PubMed).

Tao-Cheng, Thein, Yang, Reese, Gallant: "Homer is concentrated at the postsynaptic density and does not redistribute after acute synaptic stimulation." in: Neuroscience, Vol. 266, pp. 80-90, (2014) (PubMed).

Target Details

Target: Homer 1b/c

Background: Synonyms: VesL 1, Syn47