Abeta-pE3 antibody

Catalog No. ABIN1742433

Product Details

Target: Abeta-pE3

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Specificity: Specific for Abeta-pE3.

Purification: purified IgG. Azide was added before lyophilization.

Immunogen: Synthetic peptide pyr-EFRHD coupled to key-hole limpet hemocyanin via an added C-terminal cysteine residue. Epitope Minimal epitope: pyr-EFR.

Clone: 1-57

Isotype: IgG2b

Application Details

Application Notes: WB: 1 : 1000
ICC: not tested yet
IHC: 1 : 100

Comment:

WB: Detects purified Abeta pE3. Complex samples like brain extracts still have to be tested.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 µL H2O to get a 1mg/ml solution of antibody in PBS. Then aliquot and store at -20 °C until use.

Buffer: PBS, 0.02% sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C

Storage Comment: Unlabeled antibodies are stable in this form without loss of quality at ambient temperatures for several weeks or even months. They can be stored at 4 °C for several years.

References

Antonios, Saiepour, Bouter, Richard, Paetau, Verkkoniemi-Ahola, Lannfelt, Ingelsson, Kovacs, Pillot, Wirths, Bayer: "N-truncated Abeta starting with position four: early intraneuronal accumulation and rescue of toxicity using NT4X-167, a novel monoclonal antibody." in: Acta neuropathologica communications, Vol. 1, Issue 1, pp. 56, (2013) (PubMed).

Wittnam, Portelius, Zetterberg, Gustavsson, Schilling, Koch, Demuth, Blennow, Wirths, Bayer: "Pyroglutamate amyloid β (Aβ) aggravates behavioral deficits in transgenic amyloid mouse model for Alzheimer disease." in: The Journal of biological chemistry, Vol. 287, Issue 11, pp. 8154-62, (2012) (PubMed).

Target Details

Target: Abeta-pE3

Background: Synonyms: pyro-Glu Abeta, pE-Abeta, pGlu-Abeta, Abeta(3(pE)-40/42)