MAPT antibody (N-Term)

Catalog No. ABIN1742567

There are 2+ publications for this product available. The Mouse Monoclonal anti-MAPT antibody is suitable to detect MAPT in samples from Human, Rat and Mouse. It has been validated for WB, IHC, IP, ICC and IHC (p).

Quick Overview for MAPT antibody (N-Term) (ABIN1742567)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivity: Human, Rat, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 248E5

Product Details anti-MAPT Antibody

Binding Specificity: N-Term

Purpose: Mouse anti-Tau IgG

Specificity: Specific for tau.

Cross-Reactivity (Details): Reacts with: rat (P19332), mouse (P10637). Weaker signal: human (P10636). No signal: zebrafish. Other species not tested yet.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to the N-terminal half of mouse Tau-D (UniProt Id: P10637-5)

Isotype: IgG2a

Application Details

Application Notes: WB:1 : 1000 , IP:yes ICC:1 : 200 up to 1 : 500, IHC:1 : 100, IHC-P:1 : 500,

Comment:

WB: Detects the mouse protein with much greater sensitivity than the rat protein. The antibody binds phosphorylated and non-phosphorylated tau proteins.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Buffer: PBS. Albumin and azide were added for stabilization.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze! Antibodies should be stored at +4°C when still lyophilized.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Albumin and azide were added for stabilization. Do not freeze!

References for anti-MAPT antibody (ABIN1742567)

Gut, Beske, Hubbard, Lyman, Hamilton, McNutt: "Novel application of stem cell-derived neurons to evaluate the time- and dose-dependent progression of excitotoxic injury." in: PLoS ONE, Vol. 8, Issue 5, pp. e64423, (2013) (PubMed).

Ahmad, Wolber, Eckardt, Koch, Schmitt, Semechkin, Geis, Heckmann, Brüstle, McLaughlin, Sirén, Müller: "Functional neuronal cells generated by human parthenogenetic stem cells." in: PLoS ONE, Vol. 7, Issue 8, pp. e42800, (2012) (PubMed).

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: Tau

Background:

Synonyms: Microtubule-associated protein tau, Mapt, MAPT

Antigen Information: Tau is a neuronal microtubule associated protein

UniProt: P10637

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size