Recombinant PI-9 produced in P. pastoris. Spleen cells from immunised Balb/c mice were fused with cells of the mouse NS-1myeloma cell line. Genename: PI-9
Verified
SERPINB9
Reactivity: Human
WB, ELISA
Host: Goat
Polyclonal
unconjugated
Application Notes
Western Blot: This antibody detects a band of approx. 42 kda under Reducing ConditionsFlow Cytometry: Use 10 μL of 1/10-1/100 diluted antibody to label 1x10^6 cells in 100 μL. Membrane permeabilisation is required for this application (The use of Leucoperm isrecommended for this purpose). Immunohistochemistry on Paraffin Sections: 1/50. This product requires antigen retrieval using heat treatment prior to staining of paraffinsections. Sodium citrate buffer pH 6.0 is recommended for this purpose. Recommended Positive Control: Tonsil. Also Suitable for use in Indirect Immunofluorescence. (See Ref.2 for more details) Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions
For Research Use only
Concentration
1.0 mg/mL
Buffer
PBS, 0.05 % Sodium Azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.
Expiry Date
12 months
Target
SERPINB9
(serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 9 (SERPINB9))
SERPINB9 antibody, BB283241 antibody, CAP-3 antibody, CAP3 antibody, PI-9 antibody, PI9 antibody, Spi6 antibody, ovalbumin antibody, serpin family B member 9 antibody, serpin peptidase inhibitor, clade B (ovalbumin), member 9 antibody, serine (or cysteine) peptidase inhibitor, clade B, member 9 antibody, Serpinb9 antibody, SERPINB9 antibody
Background
PI-9 is a 42 kDa intracellular nucleocytoplasmic serpin expressed in cytotoxic lymphocytes (CTLs), natural killer (NK) cells, monocyte-derived dendritic cells (DCs), and to a lesser extent in B cells and myeloid cells. Granzyme B (grB) is a serine protease highly expressed by CTLs and NK cells, which is endocytosed by virusinfected and malignant target cells. The subsequent release of grB from the endocytic vesicles into the cytoplasm of the target cells, triggers grB-mediated apoptosis, through cleavage of various cytoplasmic or nuclear proteins. PI-9, up-regulated in response to grB production and degranulation, has been identified as a potent inhibitor of Granzyme B-mediated apoptosis, providing a vital self-protection mechanism against the premature apoptosis of CTLs and NK cells by grB, which may escape into the cytoplasm of the effector cells themselves.Synonyms: CAP-3, CAP3, Cytoplasmic antiproteinase 3, PI-9, PI9, Proteinase inhibitor 9, Serpin B9