BMP7 antibody (Biotin)
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- Target See all BMP7 Antibodies
- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This BMP7 antibody is conjugated to Biotin
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Application
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- Specificity
- This antibody detects BMP7.
- Purification
- Affinity chromatography
- Immunogen
- Highly pure (> 98 %) recombinant human BMP-7
- Top Product
- Discover our top product BMP7 Primary Antibody
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- Application Notes
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ELISA: Direct: To detect BMP-7 by direct ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withcompatible secondary reagents, it allows the detection of at least 0.2 - 0.4 ng/well ofrecombinant BMP-7. Sandwich: To detect BMP-7 by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.25 - 1.0 μg/mL of this antibody is required. In conjunction withPolyclonal Anti-Human BMP-7 as a capture antibody, it allows the detection of at least 0.2 -0.4 ng/well of recombinant BMP-7. Western Blot: To detect BMP-7 by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant BMP-7 is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Reconstitution
- Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/mL.
- Buffer
- PBS, pH 7.2
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Store the lyophilized antibody at -20 °C. Following reconstitution it is stable for two weeks at 2 - 8 °C. Frozen aliquots are stable for 6 months when stored at -20 °C.
- Expiry Date
- 6 months
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- Target
- BMP7 (Bone Morphogenetic Protein 7 (BMP7))
- Alternative Name
- BMP7 (BMP7 Products)
- Synonyms
- bmp-7 antibody, bmp7 antibody, op-1 antibody, BMP7 antibody, zgc:153025 antibody, OP-1 antibody, OP1 antibody, BMP-7 antibody, fb83f09 antibody, wu:fb83f09 antibody, bone morphogenetic protein 7 antibody, bone morphogenetic protein 7, gene 1 L homeolog antibody, bone morphogenetic protein 7b antibody, bone morphogenetic protein 7, gene 2 L homeolog antibody, bone morphogenetic protein 7a antibody, BMP7 antibody, bmp7.1.L antibody, LOC100136282 antibody, bmp7b antibody, Bmp7 antibody, LOC100008826 antibody, bmp7.2.L antibody, bmp7a antibody
- Background
- Bone Morphogenetic Protein 7 (BMP7), also known as osteogenic protein 1 (OP1), is produced from a DNA sequence encoding the human BMP2 signal peptide and human BMP2 propeptide (amino acid residues 1 to 282) fused to the human BMP7 mature subunit (amino acid residues 293 to 431). Mature human BMP7, generated after the proteolytic removal of the signal peptide and the propeptide, is a disulfide linked homodimeric protein, comprised of two 139 amino acid residue subunits. Bone Morphogenetic Proteins (BMP) are members of the TGF beta superfamily of cytokines that affect bone and cartilage formation. Similar to other TGF beta family proteins, BMPs are highly conserved across animal species.Synonyms: BMP-7, Bone morphogenetic protein 7, OP1, Osteogenic protein 1
- Gene ID
- 655
- NCBI Accession
- NP_001710
- UniProt
- P18075
- Pathways
- Steroid Hormone Mediated Signaling Pathway, Stem Cell Maintenance
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