ARAF antibody (AA 371-554)

Catalog No. ABIN1832873

The Rabbit Polyclonal anti-ARAF antibody has been validated for WB, IHC, IHC (p) and ICC. It is suitable to detect ARAF in samples from Human.

Quick Overview for ARAF antibody (AA 371-554) (ABIN1832873)

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARAF antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

Product Details anti-ARAF Antibody

Binding Specificity: AA 371-554

Specificity: Human ARAF / A RAF

Purification: Immunoaffinity purified

Immunogen: Recombinant fragment corresponding to a region within amino acids 371 and 554 of A-RAF (SwissProt P10398). Percent identity by BLAST analysis: Human (100%), Pig, Rat, Bovine (99%), Zebrafish (85%).

Type of Immunogen: Recombinant protein

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:100 - 1:1000), IHC, IHC-P (1:100 - 1:1000), WB (1:500 - 1:3000)

Usage: IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (  pH 6.0) or Tris-EDTA buffer (  pH 8.0).

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 0.1 M Tris-glycine,  pH 7.0, 10 % glycerol, 0.01 % Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Keep as concentrated solution. Avoid multiple freeze-thaw cycles.

Storage: -20 °C

Storage Comment: Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.

Target Details for ARAF

Target: ARAF (V-Raf Murine Sarcoma 3611 Viral Oncogene Homolog (ARAF))

Alternative Name: ARAF / ARAF1 / A-RAF

Background: Name/Gene ID: ARAF
Subfamily: Raf
Family: Protein Kinase

Synonyms: ARAF, A RAF, A-RAF, A-raf-1, PKS, PKS2, Proto-oncogene A-Raf, Proto-oncogene A-Raf-1, Ras-binding protein DA-Raf, Oncogene ARAF1, ARAF1, Proto-oncogene Pks, RAFA1

Gene ID: 369

UniProt: P10398

Pathways: MAPK Signaling, RTK Signaling, Hepatitis C