ATF4 antibody (pSer245)

Catalog No. ABIN1842329

Product Details anti-ATF4 Antibody

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Binding Specificity: pSer245

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF4 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Specificity: Rabbit Anti-ATF-4 (Phospho-Ser245) Polyclonal Antibody detects endogenous levels of ATF4 only when phosphorylated at serine 245.

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Synthesized phosphopeptide derived from human ATF-4 around pSer245 (N-R-SP-L-P)

Isotype: IgG

Application Details

Application Notes: IHC: 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year.

Target Details for ATF4

Target: ATF4 (Activating Transcription Factor 4 (Tax-Responsive Enhancer Element B67) (ATF4))

Alternative Name: ATF-4 (ATF4 Products)

Synonyms: CREB-2 antibody, CREB2 antibody, TAXREB67 antibody, TXREB antibody, ATF4 antibody, atf-4 antibody, creb-2 antibody, creb2 antibody, taxreb67 antibody, txreb antibody, atf4 antibody, wu:fj01c08 antibody, wu:fk30b04 antibody, zgc:85951 antibody, sb:eu681 antibody, wu:fb08f07 antibody, zgc:171702 antibody, Atf-4 antibody, C/ATF antibody, ATF4-I antibody, atf4-a antibody, atf4-b antibody, atf4-ii antibody, activating transcription factor 4 antibody, activating transcription factor 4 L homeolog antibody, activating transcription factor 4a antibody, activating transcription factor 4b antibody, activating transcription factor 4 S homeolog antibody, ATF4 antibody, atf4 antibody, Atf4 antibody, atf4.L antibody, atf4a antibody, atf4b antibody, atf4.S antibody

Pathways: Thyroid Hormone Synthesis, Myometrial Relaxation and Contraction, ER-Nucleus Signaling, Unfolded Protein Response