Ezrin antibody (pTyr353)

Catalog No. ABIN1842337

Product Details anti-Ezrin Antibody

Target: Ezrin (EZR)

Binding Specificity: pTyr353

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ezrin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Specificity: Rabbit Anti-Ezrin (Phospho-Tyr353) Polyclonal Antibody detects endogenous levels of Ezrin only when phosphorylated at tyrosine 353.

Characteristics: Molecular Weight: Predicted Band Size 81 kDa, Observed Band Size 81 kDa

Purification: The antibody is affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide is removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Synthesized phosphopeptide derived from human Ezrin around pTyr353 (Q-D-YP-E-E)

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:1,000,
IHC: 1:50-1:100

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: phosphate buffered saline (without Mg2+ and Ca2+), pH7.4, 150 mM NaCl, 0.02 % sodium azide, and 50 % glycerol

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.
Avoid repeated freezing and thawing.

Storage: -20 °C

Storage Comment: Store at -20 °C for 1 year.

Target Details for Ezrin

Target: Ezrin (EZR)

Alternative Name: Ezrin (EZR Products)

Synonyms: CVIL antibody, CVL antibody, VIL2 antibody, ezr antibody, vil2 antibody, wu:fc05c03 antibody, wu:fj31d06 antibody, cvil antibody, cvl antibody, Vil2 antibody, AW146364 antibody, R75297 antibody, p81 antibody, ezr.S antibody, ezrin antibody, ezrin b antibody, ezrin L homeolog antibody, EZR antibody, ezrb antibody, ezr antibody, Ezr antibody, ezr.L antibody

Pathways: Maintenance of Protein Location