CHFR antibody (C-Term)

Catalog No. ABIN184918

This Goat Polyclonal antibody specifically detects CHFR in ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for CHFR antibody (C-Term) (ABIN184918)

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This CHFR antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC)

Grade: Verified

Product Details anti-CHFR Antibody

Binding Specificity: C-Term

Purpose: CHFR

Sequence: KFNHICEQTR FKN

Specificity: This antibody is expected to recognise all reported isoforms.

Cross-Reactivity: Cow, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence KFNHICEQTRFKN, from the C Terminus of the protein sequence according to NP_001154816.1, NP_001154817.1, NP_001154818.1, NP_060693.2, NP_001154819.1.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry: In paraffin embedded Human Brain (Cerebellum) shows stained Purkinje cells. Recommended concentration: 2.5 μg/mL.

Western Blot: Preliminary experiments gave an approx 80 kDa band in human brain lysates at 0.5 μg/mL, this band was successfully blocked by incubation with the immunising peptide. Please note that currently we cannot find an explanation in the literature

Peptide ELISA: antibody detection limit dilution 1:64000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Alternative Name: CHFR

Background: CHFR, checkpoint with forkhead and ring finger domains, FLJ10796, FLJ33629, RNF116, RNF196, E3 ubiquitin-protein ligase CHFR, RING finger protein 196

Gene ID: 55743, 231600, 288734

NCBI Accession: NP_001154816, NP_001154817, NP_001154818, NP_060693, NP_001154819