HSD11B1 antibody (C-Term)

Catalog No. ABIN185329

This Goat Polyclonal antibody specifically detects HSD11B1 in WB and ELISA. It exhibits reactivity toward Human.

Quick Overview for HSD11B1 antibody (C-Term) (ABIN185329)

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This HSD11B1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-HSD11B1 Antibody

Binding Specificity: C-Term

Purpose: HSD11B1 / HDL

Sequence: CTSYNMDRFI NK

Specificity: This antibody is expected to recognise human HSD11B1 protein. Both NP_005516.1 and NP_861420.1 variants encode the same protein.

Cross-Reactivity: Cow, Human

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence CTSYNMDRFINK, from the C Terminus of the protein sequence according to NP_005516.1, NP_861420.1.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 35 kDa band observed in Human Liver lysates (calculated MW of 32.4 kDa according to NP_005516.1 and NP_861420.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:8000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for HSD11B1

Target: HSD11B1 (Hydroxysteroid (11-Beta) Dehydrogenase 1 (HSD11B1))

Alternative Name: HSD11B1

Background: HSD11B1, HDL, 11-DH, HSD11L, MGC13539, 11-beta-HSD1, hydroxysteroid (11-beta) dehydrogenase 1, corticosteroid 11-beta-dehydrogenase isozyme 1, HSD11, HSD11B, SDR26C1, 11-beta-hydroxysteroid dehydrogenase 1, OTTHUMP00000034650, short chain dehydrogenase/re

Gene ID: 3290

NCBI Accession: NP_005516, NP_861420

Pathways: Metabolism of Steroid Hormones and Vitamin D, Steroid Hormone Biosynthesis, Regulation of Carbohydrate Metabolic Process