GRK2 antibody (Internal Region)

Catalog No. ABIN185583

The Goat Polyclonal anti-GRK2 antibody (ABIN185583) specifically detects GRK2 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for GRK2 antibody (Internal Region) (ABIN185583)

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This GRK2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-GRK2 Antibody

Binding Specificity: Internal Region

Purpose: BARK1 / GRK2

Sequence: RDAYREAQQL VQR

Cross-Reactivity: Cow, Dog, Human, Mouse, Pig, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-RDAYREAQQLVQR, from the internal region of the protein sequence according to NP_001610.2.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 80 kDa band observed in Daudi cell lysates (calculated MW of 79.7 kDa according to NP_001610.1). An additional band of unknown identity was observed in some lysates at 48 kDa and was successfully blocked by incubation with the immunizi

Peptide ELISA: antibody detection limit dilution 1:16000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for GRK2

Target: GRK2 (ADRBK1) (Adrenergic, Beta, Receptor Kinase 1 (ADRBK1))

Alternative Name: GRK2

Background: BARK1, GRK2, ADRBK1, adrenergic, beta, receptor kinase 1, BETA-ARK1, beta adrenergic receptor kinase 1

Gene ID: 156, 110355

NCBI Accession: NP_001610

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of G-Protein Coupled Receptor Protein Signaling, CXCR4-mediated Signaling Events, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling