PAK1 antibody (Internal Region)

Catalog No. ABIN185685

The Goat Polyclonal anti-PAK1 antibody (ABIN185685) specifically detects PAK1 in WB and ELISA. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for PAK1 antibody (Internal Region) (ABIN185685)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reactivity: Human, Rat, Mouse

Host: Goat

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Grade: Verified

Product Details anti-PAK1 Antibody

Binding Specificity: Internal Region

Purpose: PAK1

Sequence: NTEKQKKKPK MSDE

Specificity: No cross-reactivity exptected with PAK3

Cross-Reactivity: Dog, Human, Mouse, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-NTEKQKKKPKMSDE, from the internal region of the protein sequence according to NP_002567.3.

Isotype: IgG

Application Details

Application Notes:

Western Blot: Approx 65 kDa band observed in Human Brain (Cerebral Cortex and Hippocampus) lysates (calculated MW of 60.6 kDa according to NP_002567.3). Recommended concentration: 0.5-1.5 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1

Background: PAK1, p21/Cdc42/Rac1-activated kinase 1 (STE20 homolog, yeast) , MGC130000, MGC130001, PAKalpha , p21-activated kinase 1, p21/Cdc42/Rac1-activated kinase 1 (yeast Ste20-related)

Gene ID: 5058, 18479, 29431

NCBI Accession: NP_002567

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis