HIST1H2AH antibody (AA 1-128) (Biotin)

Catalog No. ABIN1857831

Product Details anti-HIST1H2AH Antibody (Biotin)

Target: HIST1H2AH (Histone H2A Type 1-H (HIST1H2AH))

Binding Specificity: AA 1-128

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H2AH antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Sequence: MRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQ GPLGSEF- MSGRGKQGGK ARAKAKTRSS RAGLQFPVGR VHRLLRKGNY AERVGAGAPV YLAAVLEYLT AEILELAGNA ARDNKKTRII PRHLQLAIRN DEELNKLLGK VTIAQGGVLP NIQAVLLPKK TESHHKAK

Specificity: It has been selected for its ability to recognize HIST1H2AH in immunohistochemical staining and Western blotting.

Purification: Affinity Chromatography

Immunogen: Recombinant 1H2AH expressed in E.coli.
The antibody is a rabbit polyclonal antibody raised against HIST1H2AH conjugated to biotin.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.

Comment:

Content: The quality control contains recombinant 1H2AH (Met1~Lys128) disposed in loading buffer.
Usage: 10 µL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5 µL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control.Not used for other purposes.
Loading Buffer: 100 mM Tris(pH8.8), 2 % SDS, 200 mM NaCl, 50 % glycerol,BPB 0.01 % , NaN3 0.02 % .

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02 % NaN3, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze/thaw cycles

Storage: 4 °C

Storage Comment: Store at 2-8 °C for one month. Aliquot and store at -80 °C for 12 months.

Expiry Date: 12 months

Target Details for HIST1H2AH

Target: HIST1H2AH (Histone H2A Type 1-H (HIST1H2AH))

Alternative Name: Histone Cluster 1, H2ah (HIST1H2AH Products)

Synonyms: H2A/S antibody, H2AFALii antibody, H2AH antibody, dJ86C11.1 antibody, histone cluster 1 H2A family member h antibody, histone cluster 1, H2ah antibody, histone cluster 1 H2A family member H antibody, HIST1H2AH antibody, Hist1h2ah antibody