HIST1H2AB antibody (AA 2-130)

Catalog No. ABIN1859157

Product Details anti-HIST1H2AB Antibody

Target: HIST1H2AB (Histone Cluster 1, H2ab (HIST1H2AB))

Binding Specificity: AA 2-130

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H2AB antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Purpose: Polyclonal Antibody to Histone Cluster 1, H2ab (HIST1H2AB)

Specificity: The antibody is a rabbit polyclonal antibody raised against HIST1H2AB. It has been selected for its ability to recognize HIST1H2AB in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Histone Cluster 1, H2ab (HIST1H2AB) corresdonding to Ser2~Lys130 (Accession # P04908) with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes:

Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, pH 7.4, containing 0.01 % SKL, 1 mM DTT, 5 % Trehalose and Proclin300.

Preservative: Dithiothreitol (DTT), ProClin

Precaution of Use: WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for HIST1H2AB

Target: HIST1H2AB (Histone Cluster 1, H2ab (HIST1H2AB))

Alternative Name: Histone Cluster 1, H2ab (HIST1H2AB Products)

Synonyms: H2A/m antibody, H2AFM antibody, H2A.1 antibody, H2a-53 antibody, histone cluster 1 H2A family member b antibody, histone cluster 1, H2ab antibody, HIST1H2AB antibody, Hist1h2ab antibody

Background: HIST1H2AE, H2AFM, H2A/m, H2A Histone Family Member M

Pathways: Telomere Maintenance