LAG3 antibody (AA 244-487)
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- Target See all LAG3 Antibodies
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
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Binding Specificity
- AA 244-487
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This LAG3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- Purpose
- Polyclonal Antibody to Lymphocyte Activation Gene 3 (LAG3)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against LAG3. It has been selected for its ability to recognize LAG3 in immunohistochemical staining and western blotting.
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Lymphocyte Activation Gene 3 (LAG3) corresdonding to Thr244~Glu487 with N-terminal His Tag
- Isotype
- IgG
- Top Product
- Discover our top product LAG3 Primary Antibody
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- Application Notes
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Western blotting: 0.5-2 μg/mL
1:330-1300 Immunohistochemistry: 5-20 μg/mL
1:33-130 Immunocytochemistry: 5-20 μg/mL
1:33-130 Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
- Alternative Name
- Lymphocyte Activation Gene 3 (LAG3 Products)
- Synonyms
- CD223 antibody, LAG-3 antibody, Ly66 antibody, lymphocyte activating 3 antibody, lymphocyte-activation gene 3 antibody, LAG3 antibody, Lag3 antibody
- Background
- CD223, Protein FDC
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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